Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis.

Caveolae is specialized domains of the plasma membrane. Invaginations formation is dependent on the expression of Caveolin-1 or -3 and protein from the family Cavin. In response to stress, caveolae dismantle and cavins released from caveolae, allowing cavins potentially interact with intracellular targets. 

Here, we describe the intracellular (non-plasma membrane) Purified Recombinant Proteins

 Cavin interactome using biotin affinity proteomics and mass spectrometry. We validate 47-interactor protein Cavin potential using cell-free expression system and the proteins that bind to the test. These data, together with analysis of pathways, revealing unknown role for the protein Cavin in metabolism and stress signals.

 We validated the interaction between a candidate interactor protein, protein phosphatase 1 alpha (PP1α), and Cavin-1 and -3 and showed that UV treatment causes release of caveolae Cavin3 enable interaction with and inhibition, PP1α. H2AX phosphorylation increased interaction to stimulate apoptosis, identify pro-apoptotic signaling pathways from the core to the surface caveolae.

The effects of animal or plant protein diet on cecal fermentation in guinea pigs (Cavia porcellus), rat (Rattus norvegicus) and chicks (Gallus gallus domesticus).

monogastric herbivores such as guinea pig dependent on energy supplies from enteric fermentation as short chain fatty acids (SCFA) corresponds to 30-40% of their maintenance energy requirements. They develop a special digestive system to adapt their indigenous microflora on plant polysaccharide fermentation. No information was available about the adaptation of microbial fermentation in the hindgut of monogastric herbivore's diet of animal protein. 

We investigated if the guinea pig is fully able to Viral Recombinant Proteins take the energy from the diet of animal protein by hindgut fermentation compared with vegetable protein diet. For comparison, we also studied the two omnivores. end products in vitro cecal fermentation (SCFA, ammonia and gas) were measured to assess how well an animal protein diet could be fermented.

 Animal protein diet resulted in less intensive fermentation with increased feed consumption and cecal contents volume of the only plant protein diet in guinea pigs. This may be due to the limited capacity of the hindgut microflora to adapt to the animal protein-rich substrate. We also found that girls cecal contents produced methane at a rate higher emissions than ruminants.