Skip to main content

Phasin proteins activate Aeromonas caviae polyhydroxyalkanoate (PHA) synthase but not Ralstonia eutropha PHA synthase.

In this study, we performed in vitro and in vivo activity assays of polyhydroxyalkanoate (PHA) synthase (PhaCs) in the presence of protein phasin (PhaPs), which revealed that PhaPs are activators of PHAC derived from Aeromonas caviae (PhaCAc). In an in vitro study, among the three PhaCs tested, PhaCAc significantly activated when PhaPs added at the beginning of polymerization (prepolymerization PhaCAc), while prepolymerization PhaCRe (derived from Cupriavidus necator) and PhaCDa (Delftia acidovorans) showed decreased activity with PhaPs.

 The PhaCAc Phap-enabled show a slight shift in substrate preference towards 3-hydroxyhexanoyl-CoA (C6). PhaPAc also enabled PhaCAc when added during the polymerization (polymer-elongating PhaCAc), while this effect was not observed for PhaCRe.

 In an in vivo test using Escherichia coli strain Caprine Recombinant Proteins TOP10 as the host, the effect of PhaPAc expression on PHA synthesis by PhaCAc or PhaCRe examined. As PhaPAc expression increases, PHA production increased by 2.3-fold in PhaCAc-expressing strain, while it increased slightly in PhaCRe-expressing strain. Taken together, these studies provide evidence that PhaPs serves as an activator for PhaCAc both in vitro and in vivo but not activate PhaCRe. This activating effect may be associated with the new role PhaPs in polymerization reactions by PhaCAc.
Phasin proteins activate Aeromonas caviae polyhydroxyalkanoate (PHA) synthase but not Ralstonia eutropha PHA synthase.

bacterial peritonitis in the inferior vena cava liver disease: a hypothesis to explain the cause of infection in high-protein ascites.


IVC liver disease (HVD), a disease caused by complete obstruction or stenosis of the near-atrial junction cava inferior vena cava (IVC) is endemic in Nepal. It is a chronic disease characterized by upper abdominal pain, hepatomegaly, splenomegaly and dilated surface veins in the trunk of the body. Ascites generally, with a high protein Cavia Recombinant Proteins content is a feature of acute and subacute phase and during acute exacerbations of chronic disease. 

We assess the occurrence of bacterial peritonitis among patients with ascites HVD. One hundred sixty-seven consecutive patients with ascites, including 91 patients with HVD examined for the presence of bacterial peritonitis. Ascites fluid examined for total and differential WBC counting. Fluid and blood were cultured for aerobic microorganisms at the bedside inoculation in blood culture bottles. HVD is a common cause of non-cirrhotic ascites the high protein content in Nepal.
Labels:

Comments

Post a Comment

Popular posts from this blog

Cavin family proteins and the assembly of caveolae.

Caveolae is abundant features of the plasma membrane in many cells. Until now, they are generally considered invaginasi membrane formation is primarily driven by integral membrane proteins called caveolins.  However, the last decade has seen the emergence of family Cavin peripheral membrane protein as an important component coat and Yeast Recombinant Proteins  regulators of biogenesis caveola. In this Commentary, we summarize recent data on the role in the formation caveola cavins, highlighting structural studies provide new insights into the mantle Cavin assembly. In mammals, there are four members of the family Cavin association through homo and hetero-oligomerisation to form different subcomplexes on caveolae, which can be released into the cell in response to stimuli.  Studies from several laboratories have provided a better understanding of the stoichiometric Cavin and the molecular basis for their oligomerisation, as well as identifying the interaction with the membrane phosphol
Post a Comment
Read more

CAVIN-3 regulates circadian period length and PER:CRY protein abundance and interactions.

n mammals, transcriptional autorepression by Period (PER) and cryptochrome (CRY) protein complex is essential for the generation of circadian rhythms. We have identified Cavin-3, PER2-new cytoplasmic proteins interact affecting the properties of the circadian clock. Thus, Cavin-3 loss- and gain-of-function shortened and extended, respectively, in the circadian period of fibroblasts and exposed PER: CRY protein abundance and interactions.  W hile the depletion of protein kinase Cδ (PKCδ) , the pair are known Equine Recombinant Proteins  from Cavin-3, have little effect on circadian gene expression, Cavin-3 PKCδ binding sites required to exert its effect on the length of the period. This suggests the involvement of yet uncharacterized protein kinase. Finally, Cavin-3 activity in circadian gene expression is independent of caveolae. Dieckol, a phlorotannin isolated from brown seaweed, Ecklonia cava, inhibits adipogenesis through AMP-activated protein kinase (AMPK) activation in 3T3-L1 pre
Post a Comment
Read more

Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis.

Caveolae is specialized domains of the plasma membrane. Invaginations formation is dependent on the expression of Caveolin-1 or -3 and protein from the family Cavin. In response to stress, caveolae dismantle and cavins released from caveolae, allowing cavins potentially interact with intracellular targets.  Here, we describe the intracellular (non-plasma membrane) Purified Recombinant Protein s  Cavin interactome using biotin affinity proteomics and mass spectrometry. We validate 47-interactor protein Cavin potential using cell-free expression system and the proteins that bind to the test. These data, together with analysis of pathways, revealing unknown role for the protein Cavin in metabolism and stress signals.  We validated the interaction between a candidate interactor protein, protein phosphatase 1 alpha (PP1α), and Cavin-1 and -3 and showed that UV treatment causes release of caveolae Cavin3 enable interaction with and inhibition, PP1α. H2AX phosphorylation increased interacti
Post a Comment
Read more