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CAVIN-3 regulates circadian period length and PER:CRY protein abundance and interactions.

n mammals, transcriptional autorepression by Period (PER) and cryptochrome (CRY) protein complex is essential for the generation of circadian rhythms. We have identified Cavin-3, PER2-new cytoplasmic proteins interact affecting the properties of the circadian clock. Thus, Cavin-3 loss- and gain-of-function shortened and extended, respectively, in the circadian period of fibroblasts and exposed PER: CRY protein abundance and interactions. 

While the depletion of protein kinase Cδ (PKCδ), the pair are known Equine Recombinant Proteins from Cavin-3, have little effect on circadian gene expression, Cavin-3 PKCδ binding sites required to exert its effect on the length of the period. This suggests the involvement of yet uncharacterized protein kinase. Finally, Cavin-3 activity in circadian gene expression is independent of caveolae.
CAVIN-3 regulates circadian period length and PER:CRY protein abundance and interactions.

Dieckol, a phlorotannin isolated from brown seaweed, Ecklonia cava, inhibits adipogenesis through AMP-activated protein kinase (AMPK) activation in 3T3-L1 preadipocytes.


In this study, we assessed the potential inhibitory effect of 5 species of brown seaweed on adipogenesis differentiation of 3T3-L1 preadipocytes into mature adipocytes by measuring Oil-Red O staining. Ecklonia cava extract tested here proved deep adipogenesis inhibitory effect compared to that shown by the four other brown seaweed extract.

 Thus, E. cava selected for isolation of the active compound and finally three of phlorotannins derived polyphenolic compounds and inhibitory effect on adipogenesis observed. Among phlorotannins, dieckol exhibited the greatest potential inhibition of adipogenesis and down-regulated the expression of peroxisome proliferator-activated receptor-γ (PPAR?), CCAAT / enhancer binding protein (C / EBPα), sterol regulatory element-binding Fruit fly Recombinant Proteins protein 1 (SREBP1) and acid fat binding protein 4 (FABP4) with a dose-dependent manner.

 Specific mechanisms mediating the effects dieckol confirmed by AMP-activated protein kinase (AMPK) activation. These results demonstrate the inhibitory effect of compounds on adipogenesis dieckol through activation of AMPK signaling pathway.

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