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Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.

Caveolin-1 and caveolae differently polarized in cell migration in a variety of models, and Caveolin-1 expression has been shown to modulate cell migration quantitatively. PTRF / Cavin-1 is a cytoplasmic protein is now established to be also required for the formation of caveola. Here we test the effects of PTRF expression on cell migration. 

Using fluorescence imaging, quantitative General Recombinant Proteins proteomics, and cell migration tests we showed that PTRF / Cavin-1 polarization modulated cellular and subcellular localization of Rac1 and Caveolin-1 in cell migration and recruitment PKCα caveola. 

PTRF / Cavin-1 quantitative cell migration is reduced, and induced epithelial mesenchymal refund. Similar to Caveolin-1, polarization PTRF / Cavin-1 depending on the mode of migration. By selectively manipulating PTRF / Cavin-1 and Caveolin-1 expression (and therefore the formation of caveola) in several cell systems, we uncover caveola-independent functions for both proteins in cell migration.

Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.

Down-regulation of protein Cavin breast cancer families.


Caveolae is abundant membrane domain on the cell surface of various types of mammalian cells and is involved in various physiological processes. Structural proteins caveolae Caveolin-1 is frequently mutated or deregulated in cancer, and family Cavin response serum protein deficiency-related factor gene product that binds to the C-kinase (SRBC) was found epigenetically active in the lung, breast, and stomach cancer.

Both Caveolin-1, and SRBC have been proposed https://gentaur.fr/ to function as a tumor suppressor. Polymerase Factor 1 and transcript release (PTRF) is an essential component for the formation of caveolae. Regulation of the expression of cancer PTRF not been characterized. We report here that protein family Cavin PTRF, SRBC response and serum protein deficiencies down regulated in breast cancer cell lines and breast tumor tissue. 

We further showed that down-regulation of PTRF in breast cancer cells is associated with methylation of the promoter. As Caveolin-1 and Cavin family of proteins required for caveolae formation and function, tumor suppression function of Caveolin-1 is reported and SRBC may be due to the deregulation of caveolae and the downstream signaling. Thus, caveolae is a potential therapeutic target and the expression of protein Cavin family can be a useful prognostic indicator of breast cancer development.

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